SONG Shijie，WANG Yi，PENG Ruisi，et al. Effects of coal mining subsidence on soil microorganisms and enzyme activities in different landform types of northern Shaanxi[J]. Coal Science and Technology，2023，51（12）：110−124
. DOI: 10.12438/cst.2023-0924| Citation: |
SONG Shijie，WANG Yi，PENG Ruisi，et al. Effects of coal mining subsidence on soil microorganisms and enzyme activities in different landform types of northern Shaanxi[J]. Coal Science and Technology，2023，51（12）：110−124 . DOI: 10.12438/cst.2023-0924
|
A profound understanding of the derivative ecological damage effects of coal mining subsidence has become a key basic scientific issue and research hotspot for repairing ecological environment damage caused by mining in western coal mining areas. Grasping how coal mining subsidence in different geomorphic units affects soil microorganisms and enzyme activities is beneficial for the ecological environment protection and restoration of coal mining subsidence areas in the western region. The typical subsidence slope soil of the Yushuwan mine field (aeolian landform) and the north wing of the Ningtiaota mine field (loess landform) in northern Shaanxi coal mine area were selected as the research objects, while the soil samples from different slope parts (vertical depth of 0~60 cm) were collected, respectively. Absolute quantitative PCR and enzyme-linked immunosorbent assay (ELISA) methods were used to determine the number of soil bacteria, actinomycetes and fungi, as well as the activities of sucrase, catalase, urease and phosphatase, respectively. The spatial variation characteristics of soil microbial quantity and enzyme activity on subsidence slope were thoroughly analyzed, while the main physical and chemical soil properties were comprehensively integrated to reveal the impact of coal mining subsidence on soil microorganism and enzyme activity under different landform types. The results showed that: ① Coal mining subsidence in both aeolian landform and loess landform type unit could significantly reduce the number of soil microorganisms and enzyme activity on the subsidence slope. The decrease in soil microbial quantity reached 8.27%-42.39% and 11.53%-45.95%, respectively, while the decrease in soil enzyme activity reaches 6.52%-39.83% and 9.09%-42.42%, respectively. With the transition from the top of the slope to the middle of the slope and then to the foot of the slope, the reduction effect exhibited different variation characteristics for three soil microorganisms and four enzymes; ② The coal mining subsidence of loess landform type unit performed the most significant reduction effect on various soil microbial quantity and enzyme activity in “middle part of slope 0~10 cm soil layer”. Meanwhile, the coal mining subsidence of aeolian landform unit presented the most significant reduction effects on the soil actinomycetes quantity and invertase, urease and phosphatase activities in “middle part of slope 0~10 cm soil layer”, and soil bacteria and fungi quantity in “middle part of slope 10~20 cm soil layer”, as well as soil catalase activity in “middle part of slope 20~40 cm soil layer”. Therefore, it can be served as a targeted area for slope soil microbial remediation in coal mining subsidence areas of northern Shaanxi; ③ Whether in the units of aeolian landform or loess landform, the number of soil fungi and urease activity were the most sensitive indicators to coal mining subsidence, with decreases of 23.28%-45.95% and 22.78%-42.42%, respectively. Moreover, the two indicators can be used as markers to analyze the microbial characteristics of soil damaged by coal mining subsidence in northern Shaanxi mining area; ④ The correlation coefficients between soil available phosphorus and organic matter content and soil microbes and enzyme activities were the highest, exceeding 0.8 and 0.7, respectively, on both wind-sand and loess subsidence slopes. They can be served as key indicator factors for coal mining subsidence affecting soil microbial characteristics.
| [1] |
王双明,申艳军,宋世杰,等. “双碳”目标下煤炭能源地位变化与绿色低碳开发[J]. 煤炭学报,2023,48(7):2599−2612.
WANG Shuangming,SHEN Yanjun,SONG Shijie, et al. Change of coal energy status and green and low-carbon development under the“dual carbon”goal[J]. Journal of China Coal Society,2023,48(7):2599−2612.
|
| [2] |
国家统计局. 中华人民共和国2022年国民经济和社会发展统计公报[EB/OL]. (2023-02-28)[2023-08-01] http://www.stats.gov.cn/sj/zxfb/202302/t20230228_1919011.html.
|
| [3] |
王双明,魏江波,宋世杰,等. 黄河流域陕北煤炭开采区厚砂岩对覆岩采动裂隙发育的影响及采煤保水建议[J]. 煤田地质与勘探,2022,50(12):1−11.
WANG Shuangming,WEI Jiangbo,SONG Shijie, et al. Influence of thick sandstone on development of overburden mining fissures in northern Shaanxi coal mining area of Yellow River Basin and suggestions on water-preserved coal mining[J]. Coal Geology & Exploration,2022,50(12):1−11.
|
| [4] |
宋世杰,孙 涛,杜 麟,等. 陕北煤矿区不同形态的采动地裂缝对土壤可蚀性的影响[J/OL]. 煤炭学报:1−14[2023-05-23]. http://kns.cnki.net/kcms/detail/11.2190.td.20230303.1533.003.html.
SONG Shijie,SUN Tao,DU Lin, et al. Effects of mining ground fissures of different shapes on soil erodibility in Northern Shaanxi Coal Mining Area influence[J/OL]. Journal of China Coal Society:1−14[2023-05-23]. http://kns.cnki.net/kcms/detail/11.2190.td.20230303.1533.003.html.
|
| [5] |
宋世杰,孙 涛,郑贝贝,等. 陕北黄土沟壑区采煤沉陷对黄土坡面形态的影响及土壤侵蚀效应[J]. 煤炭科学技术,2023,51(2):422−435.
SONG Shijie,SUN Tao,ZHENG Beibei, et al. Effect of coal mining subsidence on loess slope morphology and soil erosion in loess gully region of Northern Shaanxi[J]. Coal Science and Technology,2023,51(2):422−435.
|
| [6] |
宋世杰,王双明,赵晓光,等. 基于覆岩层状结构特征的开采沉陷分层传递预计方法[J]. 煤炭学报,2018,43(S1):87−95.
SONG Shijie,WANG Shuangming,ZHAO Xiaoguang, et al. Stratification transfer method of the mining subsidence based on the characteristics of layered structure in coal overburden[J]. Journal of China Coal Society,2018,43(S1):87−95.
|
| [7] |
宋世杰,杜 麟,王双明,等. 陕北采煤沉陷区不同沉陷年限黄土坡面土壤可蚀性的变化规律[J]. 煤炭科学技术,2022,50(2):289−299.
SONG Shijie,DU Lin,WANG Shuangming, et al. Variation of soil erodibility on loess under various subsidence years in coal mining subsidence area located Northern Shaanxi[J]. Coal Science and Technology,2022,50(2):289−299.
|
| [8] |
包玉英,马晓丹,赵旭鹏,等. 露天煤炭开采对矿坑周围土壤生物活性的影响[J]. 煤炭科学技术,2020,48(4):89−96.
BAO Yuying,MA Xiaodan,ZHAO Xupeng, et al. Effect of open-pit coal mining on soil bio-activity around the pit[J]. Coal Science and Technology,2020,48(4):89−96.
|
| [9] |
毕银丽,胡晶晶,刘 京. 煤矿微生物复垦区灌木林下土壤养分的空间异质性[J]. 煤炭学报,2020,45(8):2908−2917.
BI Yinli,HU Jingjing,LIU Jing. Spatial heterogeneity of soil nutrients under shrubbery in micro-reclamation demonstration base in coal mine areas of China[J]. Journal of China Coal Society,2020,45(8):2908−2917.
|
| [10] |
肖浩宇. 开采沉陷对土壤关键物理性质影响规律研究[D]. 徐州: 中国矿业大学,2018.
XIAO Haoyu. Study on the influence of mining subsidence on the key physical properties of soil[D]. Xuzhou: China University of Mining and Technology,2018.
|
| [11] |
王双明,杜华栋,王生全. 神木北部采煤塌陷区土壤与植被损害过程及机理分析[J]. 煤炭学报,2017,42(1):17−26.
WANG Shuangming,DU Huadong,WANG Shengquan. Analysis of damage process and mechanism for plant community and soil properties at northern Shenmu subsidence mining area[J]. Journal of China Coal Society,2017,42(1):17−26.
|
| [12] |
胡振琪,陈 超. 风沙区井工煤炭开采对土地生态的影响及修复[J]. 矿业科学学报,2016,1(2):120−130.
HU Zhenqi,CHEN Chao. Impact of underground coal mining on land ecology and its restoration in windy and sandy region[J]. Journal of Mining Science and Technology,2016,1(2):120−130.
|
| [13] |
卞正富,雷少刚,刘 辉,等. 风积沙区超大工作面开采生态环境破坏过程与恢复对策[J]. 采矿与安全工程学报,2016,33(2):305−310.
BIAN Zhengfu,LEI Shaogang,LIU hui, et al. The process and countermeasures for ecological damage and restoration in coal mining area with super-size mining face at aeolian sandy site[J]. Journal of Mining & Safety Engineering,2016,33(2):305−310.
|
| [14] |
宋世杰,张玉玲,王双明,等. 陕北煤矿区采动地裂缝对土壤微生物和酶活性的影响[J]. 煤炭学报,2021,46(5):1630−1640.
SONG Shijie,ZHANG Yuling,WANG Shuangming, et al. Influence of mining ground fissures on soil microorganism and enzyme activities in northern Shaanxi coal mining area[J]. Journal of China Coal Society,2021,46(5):1630−1640.
|
| [15] |
VISHWAKARMA A K,BEHERA T,RAI R, et al. Impact assessment of coal mining induced subsidence on native soil of South Eastern Coal Fields:India[J]. Geomechanics and Geophysics for Geo-Energy and Geo-Resources,2020,6(1):1−21. doi: 10.1007/s40948-019-00123-2
|
| [16] |
宋子恒,刘 刚,孙丽平,等. 采煤扰动对土壤理化性状影响及微生物群落响应机制[J]. 煤田地质与勘探,2023,51(3):95−102.
SONG Ziheng,LIU Gang,SUN Liping, et al. Influence of coal mining disturbance on soil physiochemical properties and the response mechanism of microbial community[J]. Coal Geology & Exploration,2023,51(3):95−102.
|
| [17] |
李智兰. 矿区复垦对土壤养分和酶活性以及微生物数量的影响[J]. 水土保持通报,2015,35(2):6−13.
LI Zhilan. Soil nutrients,enzyme activity and microbe quantity in reclaimed soil in mining area[J]. Bulletin of Soil and Water Conservation,2015,35(2):6−13.
|
| [18] |
马守臣,张合兵,王 锐,等. 煤矸石填埋场土壤微生物学特性的时空变异[J]. 煤炭学报,2015,40(7):1608−1614.
MA Shouchen,ZHANG Hebing,WANG Rui, et al. Spatial-temporal variation of soil microbial characteristics in coal gangue field[J]. Journal of China Coal Society,2015,40(7):1608−1614.
|
| [19] |
EMMERT E A B,GELETA S B,ROSE C M, et al. Effect of land use changes on soil microbial enzymatic activity and soil microbial community composition on Maryland's Eastern Shore[J]. Applied Soil Ecology,2021,161:103824. doi: 10.1016/j.apsoil.2020.103824
|
| [20] |
马建全,吴钶桥,彭昊,等. 煤岩采动应力−裂隙带发育规律研究[J]. 西安科技大学学报,2022,42(1):107−115.
MA Jianquan,WU Keqiao,PENG Hao, et al. Development law of mining-induced stress-fracture field in coal seam overburden[J]. Journal of Xi’an University of Science and Technology,2022,42(1):107−115.
|
| [21] |
SONG S,ZHENG B,SUN T, et al. Influence of different mining damage types on soil erodibility in coal mining areas of northern shaanxi in the middle reaches of the yellow river in China[J]. Sustainability,2023,15(6):5434. doi: 10.3390/su15065434
|
| [22] |
鲁 凡. 煤矿复垦区修复重金属污染土壤的植物筛选[D]. 西安:西安科技大学,2014.
LU Fan. Screening plant for repair heavy metal contaminated soil in coal mine reclamation district[D]. Xi’an: Xi’an University of Science and Technology,2014.
|
| [23] |
台晓丽,胡振琪,陈 超. 西部风沙区不同采煤沉陷区位土壤水分中子仪监测[J]. 农业工程学报,2016,32(15):225−231.
TAI Xiaoli,HU Zhenqi,CHEN Chao. Monitoring soil moisture at different subsidence areas of mining in western windy and sandy region with neutron instrument[J]. Transactions of the Chinese Society of Agricultural Engineering,2016,32(15):225−231.
|
| [24] |
王 健. 神东煤田沉陷区生态受损特征及环境修复研究[D]. 呼和浩特:内蒙古农业大学,2017.
WANG Jian. Study on ecological characteristics and environmental restoration of subsidence areas in Shendong Coalfied[D]. Hohhot: Inner Mongolia Agricultural University,2017.
|
| [25] |
李 娜,张利敏,张雪萍. 土壤微生物群落结构影响因素的探讨[J]. 哈尔滨师范大学自然科学学报,2012,28(6):70−74.
LI Na,ZHANG Limin,ZHANG Xueping. Discussion on the influencing factors of soil microbial community structure[J]. Natural Science Journal of Harbin Normal University,2012,28(6):70−74.
|
| [26] |
万忠梅,吴景贵. 土壤酶活性影响因子研究进展[J]. 西北农林科技大学学报(自然科学版),2005,33(6):87−92.
WAN Zhongmei,WU Jinggui. Study progress on factors affecting soil enzyme activity[J]. Journal of Northwest Science and Technology University of Agriculture and Forestry (Nature Science Edition),2005,33(6):87−92.
|
| [27] |
朱义族,李雅颖,韩继刚,等. 水分条件变化对土壤微生物的影响及其响应机制研究进展[J]. 应用生态学报,2019,30(12):4323−4332.
ZHU Yizu,LI Yaying,HAN Jigang, et al. Effects of changes in water status on soil microbes and their response mechanism:a review[J]. Chinese Journal of Applied Ecology,2019,30(12):4323−4332.
|
| [28] |
王彦峰,肖 波,王 兵,等. 黄土高原水蚀风蚀交错区藓结皮对土壤酶活性的影响[J]. 应用生态学报,2017,28(11):3553−3561.
WANG Yanfeng,XIAO Bo,WANG Bing, et al. Effects of moss-dominated biological soil crusts on soil enzyme activities in water-wind erosion crisscross region on the Loess Plateau of China[J]. Chinese Journal of Applied Ecology,2017,28(11):3553−3561.
|
| [29] |
刘 远,张 辉,熊明华,等. 气候变化对土壤微生物多样性及其功能的影响[J]. 中国环境科学,2016,36(12):3793−3799.
LIU Yuan,ZHANG Hui,XIONG Minghua, et al. Effect of climate change on soil microbial diversity and function[J]. China Environmental Science,2016,36(12):3793−3799.
|
| [30] |
林 娜,刘 勇,李国雷,等. 森林土壤酶研究进展[J]. 世界林业研究,2010,23(4):21−25.
LIN Na,LIU Yong,LI Guolei, et al. Research progress on forest soil enzyme[J]. World Forestry Research,2010,23(4):21−25.
|
| [31] |
史沛丽,张玉秀,胡振琪,等. 采煤塌陷对中国西部风沙区土壤质量的影响机制及修复措施[J]. 中国科学院大学学报,2017,34(3):318−328.
SHI Peili,ZHANG Yuxiu,HU Zhenqi, et al. Infulence mechanism of coal mining subsidence on soil quality and restoration measures in west China aeolian sand area[J]. Journal of University of Chinese Academy of Sciences,2017,34(3):318−328.
|
| [32] |
黄雪琳,杨 静,贺宇纯. 土壤酶活性的主要影响因素分析[J]. 现代园艺,2018,11(6):92−93.
HUANG Xuelin,YANG Jing,HE Yuchun. Analysis of the main influencing factors on soil enzyme activity[J]. Contemporary Horticulture,2018,11(6):92−93.
|
| [33] |
DU L,WANG R,GAO X, et al. Divergent responses of soil bacterial communities in erosion-deposition plots on the Loess Plateau[J]. Geoderma,2020,358:113995. doi: 10.1016/j.geoderma.2019.113995
|
| [34] |
彭铁双. 磷添加对闽楠人工幼林土壤养分元素及微生物的影响[D]. 长沙:中南林业科技大学,2022.
PENG Tieshuang. Effects of phosphorus addition on soil nutrient elements and microorganisms in young Phoebe bournei plantations[D]. Changsha:Central South University of Forestry and Technology,2022.
|
| [35] |
刘雅淑,孟春凤,刘延鹏,等. 森林土壤磷酸酶活性变化特征及其影响因素[J]. 湖北农业科学,2016,55(4):850−854.
LIU Yashu,MENG Chunfeng,LIU Yanpeng, et al. Variations of phosphatase activity in forest soil and its influencing factors[J]. Hubei Agricultural Sciences,2016,55(4):850−854.
|
| [36] |
于建光,常志州,黄红英,等. 秸秆腐熟剂对土壤微生物及养分的影响[J]. 农业环境科学学报,2010,29(3):563−570.
YU Jianguang,CHANG Zhizhou,HUANG Hongying, et al. Effect of microbial inoculants for straw decomposing on soil microorganisms and the nutrients[J]. Journal of Agro-Environment Science,2010,29(3):563−570.
|
| [37] |
董莉丽. 不同土地利用类型下土壤水稳性团聚体的特征[J]. 林业科学,2011,47(4):95−100.
DONG Lili. Characteristics of soil water stable aggregates under different land-use types[J]. Scientia Silvae Sinicae,2010,29(3):563−570.
|
| [38] |
SCHUTTER M E,DICK R P. Microbial community profiles and activities among aggregates of winter fallow and cover-cropped soil[J]. Soil Science Society of America Journal,2002,66(1):142−153.
|
| [39] |
李忠佩,李德成,张桃林,等. 红壤水稻土肥力性状的演变特征[J]. 土壤学报,2003,40(6):870−878.
LI Zhongpei,LI Decheng,ZHANG Taolin, et al. Evolution characteristics of fertility traits of red paddy soil[J]. Acta Pedologica Sinica,2003,40(6):870−878.
|
| [40] |
杜 涛,毕银丽,邹 慧,等. 地表裂缝对沙柳根际微生物和酶活性的影响[J]. 煤炭学报,2013,38(12):2221−2226.
DU Tao,BI Yinli,ZOU Hui, et al. Effects of surface cracks caused by coal mining on microorganisms and enzyme activities in rhizosphere of Salix psammophila[J]. Journal of China Coal Society,2013,38(12):2221−2226.
|
| [41] |
吴发启,赵西宁,佘 雕. 坡耕地土壤水分入渗影响因素分析[J]. 水土保持通报,2003,21(1):16−18,78.
WU Faqi,ZHAO Xining,SHE Diao. Analysis on Affecting Factors of Soil Infiltration in Slope Farmland[J]. Bulletin of Soil and Water Conservation,2003,21(1):16−18,78.
|
| [42] |
周瑞平. 鄂尔多斯地区采煤塌陷对风沙土壤性质的影响[D]. 呼和浩特:内蒙古农业大学,2008.
ZHOU Ruiping. Influence of coal mining subsidence on properties of sandy soil in Erdos[D]. Hohhot:Inner Mongolia Agricultural University,2008.
|
| [43] |
郭京衡,李尝君,曾凡江,等. 2种荒漠植物根系生物量分布与土壤水分、养分的关系[J]. 干旱区研究,2016,33(1):166−171.
GUO Jingheng,LI Changjun,ZENG Fanjiang, et al. The relationship between root biomass distribution of two desert plants and soil moisture and nutrients[J]. Arid Zone Research,2016,33(1):166−171.
|
| [44] |
张旭阳,刘 英,龙林丽,等. 干旱半干旱区采煤沉陷引起的土壤水分变化及其对植物生理生态潜在影响分析综述[J]. 浙江大学学报(农业与生命科学版),2022,48(4):415−425.
ZHANG Xuyang,LIU Ying,LONG Linli, et al. Review on analysis of soil moisture changes caused by coal mining subsidence in arid and semi-arid areas and their potential effects on plant physiology and ecology[J]. Journal of Zhejiang University (Agriculture & Life Sciences),2022,48(4):415−425.
|
| [45] |
钱者东,秦卫华,沈明霞,等. 毛乌素沙地煤矿开采对植被景观的影响[J]. 水土保持通报,2014,34(5):299−303.
QIAN Zhedong,QIN Weihua,SHEN Mingxia, et al. Impacts of coal mining on vegetation landscape in mu Us sandland[J]. Bulletin of Soil and Water Conservation,2014,34(5):299−303.
|
| [46] |
王新静. 风沙区高强度开采土地损伤的监测及演变与自修复特征[D]. 北京:中国矿业大学(北京),2014.
WANG Xinjing. Monitoring,evolution and self-healing characteristics of land damage due to high tension coal mining in windy and sandy area[D]. Beijing: China University of Mining and Technology-Beijing,2014.
|
| [47] |
魏婷婷,胡振琪,曹远博,等. 风沙区超大工作面开采对土壤及植物特性的影响[J]. 四川农业大学学报,2014,32(4):376−381.
WEI Tingting,HU Zhenqi,CAO Yuanbo, et al. Impacts of large coal mining on soil and plant characteristics in sandy area[J]. Journal of Sichuan Agricultural University,2014,32(4):376−381.
|
| [48] |
苏 敏. 采煤塌陷区土壤养分循环及对生态环境的影响研究[D]. 邯郸:河北工程大学,2010.
SU Min. Test study on soil nutrient cycling of coal mining subsidence area and its impact on ecological environment[D]. Handan:Hebei University of Engineering,2010.
|
| [49] |
史沛丽. 采煤沉陷对西部风沙区土壤理化特性和细菌群落的影响[D]. 北京:中国矿业大学(北京),2018.
SHI Peili. The effect of coal mining subsidence on soil properties and bacterial community structure in the west aeolian sand area[D]. Beijing: China University of Mining and Technology-Beijing,2018.
|
| [50] |
张 萌. 毛乌素沙地采煤沉陷对土壤物理性质的影响[D]. 呼和浩特:内蒙古农业大学,2020.
ZHANG Meng. Effects of coal mining subsidence on soil physical properties in Mu Us sandy land[D]. Hohhot: Inner Mongolia Agricultural University,2020.
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: