MA Jing,HUA Ziyi,CHENG Yanjun,et al. Impacts of vegetation restoration type on abundant and rare microflora inreclaimed soil of open-pit mining area[J]. Coal Science and Technology,2024,52(2):363−377
. DOI: 10.12438/cst.2023-1882Citation: |
MA Jing,HUA Ziyi,CHENG Yanjun,et al. Impacts of vegetation restoration type on abundant and rare microflora inreclaimed soil of open-pit mining area[J]. Coal Science and Technology,2024,52(2):363−377 . DOI: 10.12438/cst.2023-1882 |
Vegetation restoration is crucial for improving the ecological environment of mining areas, which could promote the development of reconstructed soil, thus regulating biogeochemical cycles, and exerting ecosystem functions. Therefore, it is essential and necessary to conduct in-depth research on the impact of vegetation restoration on soil microbial communities in open-pit mining areas. In this study, surface soil samples were collected from six typical reclamation plots, including bare land (CK),Medicago sativa(GL),Hippophae rhamnoides(BL),Pinus tabulaeformis(CF),Populus tomentosa(BF), andPopulus tomentosa+Pinus tabulaeformis(MF), located in the eastern waste dump of Heidaigou mining area of Zhungeer Banner, Inner Mongolia. High throughput sequencing, co-occurrence networks, and correlation analysis were used to explore the influential mechanism of vegetation types on soil abundant, rare bacterial and fungal community structural composition and diversity. Results showed that ① there were significant differences in the effects of different vegetation restoration types on soil physicochemical properties and enzyme activity (P<0.05). The soil organic matter content, ammonium nitrogen content, and leucine aminopeptidase activity were significantly higher than those of CK. BL performed the advantage in accumulating soil organic matter, nitrate nitrogen, and available phosphorus, while the urease, leucine aminopeptidase, and alkaline phosphatase activities were significantly increased (P<0.05). ② The vegetation type has significantly affected the composition of soil abundant and rare microbial communities (P<0.05), with more abundant and rare bacterial species than fungi, whereas the variation of fungal abundance was more significant, especially the rare fungi. The Shannon index of abundant and rare bacteria, and rare fungal communities in different vegetation restoration plots was higher than that in CK, while their community structures presented the significant differences (P<0.05). ③ Different vegetation restoration types have increased the network topology parameters and complexity of abundant and rare bacteria and fungi. The amplitude test results of removing nodes to change natural connectivity indicated that BL could enhance the stability of soil abundant bacterial network, abundant and rare fungal networks, as well as their resistance to external interferences. ④ Soil URE, SOM, and ALP were the dominant factors for changes of soil microbial community structure. For the BL plot, the pH value, SOM, AP, β- Glucosidase, URE, and ALP have significantly affected the abundant and rare microbial communities (P<0.05). In a word, the BL restoration model performed a better effect on improving soil quality during the ecological reclamation process in mining areas. The research results can provide theoretical basis for the development and utilization of soil microbial resources for vegetation restoration in the damaged mining areas.
[1] |
ZHANG Q,MA J,YANG Y J,et al. Mining subsidence-induced microtopographic effects alter the interaction of soil bacteria in the sandy pasture,China[J]. Frontiers in Environmental Science,2021,9:656708. doi: 10.3389/fenvs.2021.656708
|
[2] |
陈 浮,王思遥,于昊辰,等. 碳中和目标下煤炭变革的技术路径[J]. 煤炭学报,2022,47(4):1452−1461.
CHEN Fu,WANG Siyao,YU Haochen,et al. Technological innovation paths of coal industry for achieving carbon neutralization[J]. Journal of China Coal Society,2022,47(4):1452−1461.
|
[3] |
杨云礼,徐 明,邹 晓,等. 不同植被类型对黔中山地丘陵区土壤细菌群落特征的影响研究[J]. 生态与农村环境学报,2021,37(4):518−525.
YANG Yunli,XU Ming,ZOU Xiao,et al. Effects of different vegetation types on the characteristics of soil bacterial communities in the hilly area of Central Guizhou[J]. Journal of Ecology and Rural Environment,2021,37(4):518−525.
|
[4] |
JOUSSET A,BIENHOLD C,CHATZINOTAS A,et al. Where less may be more:how the rare biosphere pulls ecosystems strings[J]. ISME Journal,2017,11(4):853−862. doi: 10.1038/ismej.2016.174
|
[5] |
刘 蕾,史建硕,张国印,等. 长期施有机肥对设施番茄土壤稀有和丰富细菌亚群落的影响[J]. 中国农业科学,2023,56(18):3615−3628. doi: 10.3864/j.issn.0578-1752.2023.18.010
LIU Lei,SHI Jianshuo,ZHANG Guoyin,et al. Effects of long-term application of organic fertilizer on rare and abundant bacterial sub-communities in greenhouse tomato soil[J]. Scientia Agricultura Sinica,2023,56(18):3615−3628. doi: 10.3864/j.issn.0578-1752.2023.18.010
|
[6] |
SINGH B K,QUINCE C,MACDONALD C A,et al. Loss of microbial diversity in soils is coincident with reductions in some specialized functions[J]. Environmental Microbiology,2014,16(8):2408−2420. doi: 10.1111/1462-2920.12353
|
[7] |
JIAO S,WANG J,WEI G,et al. Dominant role of abundant rather than rare bacterial taxa in maintaining agro-soil microbiomes under environmental disturbances[J]. Chemosphere,2019,235:248−259. doi: 10.1016/j.chemosphere.2019.06.174
|
[8] |
PEDRÓS-ALIÓ C. The rare bacterial biosphere[J]. Annual Review of Marine Science,2011,4(1):449−466.
|
[9] |
CHEN Q L,DING J,ZHU D,et al. Rare microbial taxa as the major drivers of ecosystem multifunctionality in long-term fertilized soils[J]. Soil Biology and Biochemistry,2020,141:107686. doi: 10.1016/j.soilbio.2019.107686
|
[10] |
YANG Y,CHAI Y,XIE H,et al. Responses of soil microbial diversity,network complexity and multifunctionality to three land-use changes[J]. Science of the Total Environment,2023,859:160255. doi: 10.1016/j.scitotenv.2022.160255
|
[11] |
CHANG Y Y,CHEN F,ZHU Y F,et al. Influence of revegetation on soil microbial community and its assembly process in the open-pit mining area of the Loess Plateau,China[J]. Frontiers in Microbiology,2022,13:992816. doi: 10.3389/fmicb.2022.992816
|
[12] |
FAUST K,RAES J. Microbial interactions:from networks to models[J]. Nature Reviews Microbiology,2012,10(8):538−550. doi: 10.1038/nrmicro2832
|
[13] |
张 鹏,赵 洋,黄 磊,等. 植被重建对露天煤矿排土场土壤酶活性的影响[J]. 生态学报,2016,36(9):2715−2723.
ZHANG Peng,ZHAO Yang,HUANG Lei,et al. Effect of revegetation on soil extracellular enzyme activity in the dumping site of an open-pit coal mine in Heidaigou[J]. Acta Ecologica Sinica,2016,36(9):2715−2723.
|
[14] |
鲍士旦. 土壤农化分析[M]. 北京:中国农业出版社:2000:39−236.
|
[15] |
关松荫. 土壤酶及其研究法[M]. 北京:农业出版社:1986:274−339.
|
[16] |
MO Y,ZHANG W,YANG J,et al. Biogeographic patterns of abundant and rare bacterioplankton in three subtropical bays resulting from selective and neutral processes[J]. The ISME Journal,2018,12(9):2198−2210. doi: 10.1038/s41396-018-0153-6
|
[17] |
ZHOU X,WU F. Land-use conversion from open field to greenhouse cultivation differently affected the diversities and assembly processes of soil abundant and rare fungal communities[J]. Science of The Total Environment,2021,788:147751. doi: 10.1016/j.scitotenv.2021.147751
|
[18] |
WANG X,WANG Z,LIU W,et al. Abundant and rare fungal taxa exhibit different patterns of phylogenetic niche conservatism and community assembly across a geographical and environmental gradient[J]. Soil Biology and Biochemistry,2023,186:109167. doi: 10.1016/j.soilbio.2023.109167
|
[19] |
陈 浮,朱燕峰,马 静,等. 黄土高原矿区生态修复固碳机制与增汇潜力及调控[J]. 煤炭科学技术,2023,51(1):502−513.
CHEN Fu,ZHU Yanfeng,MA Jing,et al. Mechanism,potential and regulation of carbon sequestration and sink enhancement in ecological restoration of mining areas in the Loess Plateau[J]. Coal Science and Technology,2023,51(1):502−513.
|
[20] |
YANG M,YANG D,YU X. Soil microbial communities and enzyme activities in sea-buckthorn (Hippophae rhamnoides) plantation at different ages[J]. PLoS One,2018,13(1):e0190959. doi: 10.1371/journal.pone.0190959
|
[21] |
王 晓,毕银丽,王 义,等. 沙棘林密度和丛枝菌根真菌接种对林下植物和土壤性状的影响[J]. 林业科学,2023,59(10):138−149.
WANG Xiao,BI Yinli,WANG Yi,et al. Effects of planting density of Hippophae rhamnoides and inoculation of AMF on understory vegetation growth and soil improvement[J]. Scientia Silvae Sinicae,2023,59(10):138−149.
|
[22] |
宋世杰,张玉玲,王双明,等. 陕北煤矿区采动地裂缝对土壤微生物和酶活性的影响[J]. 煤炭学报,2021,46(5):1630−1640.
SONG Shijie,ZHANG Yuling,WANG Shuangming,et al. Influence of mining ground fissures on soil microorganism and enzyme activities in Northern Shaanxi coal mining area[J]. Journal of China Coal Society,2021,46(5):1630−1640.
|
[23] |
宁岳伟,刘 勇,张 红,等. 煤矿矿区复垦植被类型对土壤微生物功能基因和酶活的影响[J]. 环境科学,2022,43(9):4647−4654.
NING Yuewei,LIU Yong,ZHANG Hong,et al. Effects of different vegetation types on soil microbial functional genes and enzyme activities in reclaimed coal mine[J]. Environmental Science,2022,43(9):4647−4654.
|
[24] |
WU B,LUO H,WANG X,et al. Effects of environmental factors on soil bacterial community structure and diversity in different contaminated districts of Southwest China mine tailings[J]. Science of The Total Environment,2022,802:149899. doi: 10.1016/j.scitotenv.2021.149899
|
[25] |
董文雪,马 静,何 环,等. 黄淮平原矿区土地复垦对微生物群落结构和功能的影响[J]. 煤炭科学技术,2023,51(11):223−233.
DONG Wenxue,MA Jing,HE Huan,et al. Effects of land reclamation on soil microbial community structure and function in the Huang-Huai plain mining area[J]. Coal Science and Technology,2023,51(11):223−233.
|
[26] |
GUO Y F,CHENG S L,FANG H J,et al. Responses of soil fungal taxonomic attributes and enzyme activities to copper and cadmium co-contamination in paddy soils[J]. Science of the Total Environment,2022,844:157119. doi: 10.1016/j.scitotenv.2022.157119
|
[27] |
马建军,姚 虹,刘 辉,等. 燕山矿区苜蓿恢复过程中土壤养分与微生物的演变特征[J]. 环境工程技术学报,2023,13(1):270−279. doi: 10.12153/j.issn.1674-991X.20210791
MA Jianjun,YAO Hong,LIU Hui,et al. Evolution characteristics of soil nutrients and microorganisms during alfalfa restoration of mining area in Yanshan Mountain[J]. Journal of Environmental Engineering Technology,2023,13(1):270−279. doi: 10.12153/j.issn.1674-991X.20210791
|
[28] |
BAI Y C,LI B X,XU C Y,et al. Intercropping walnut and tea:effects on soil nutrients,enzyme activity,and microbial communities[J]. Frontiers in Microbiology,2022,13:852342. doi: 10.3389/fmicb.2022.852342
|
[29] |
BASTIDA F,HERNANDEZ T,ALBALADEJO J,et al. Phylogenetic and functional changes in the microbial community of long-term restored soils under semiarid climate[J]. Soil Biology & Biochemistry,2013,65:12−21.
|
[30] |
KONG J,HE Z,CHEN L,et al. Efficiency of biochar,nitrogen addition,and microbial agent amendments in remediation of soil properties and microbial community in Qilian Mountains mine soils[J]. Ecology and Evolution,2021,11(14):9318−9331. doi: 10.1002/ece3.7715
|
[31] |
LI P,ZHANG X,HAO M,et al. Effects of vegetation restoration on soil bacterial communities,enzyme activities,and nutrients of reconstructed soil in a mining area on the Loess Plateau,China[J]. Sustainability,2019,11(8):1−16.
|
[32] |
JIAO S,LU Y H. Soil pH and temperature regulate assembly processes of abundant and rare bacterial communities in agricultural ecosystems[J]. Environmental Microbiology,2020,22(3):1052−1065. doi: 10.1111/1462-2920.14815
|
[33] |
CARLTON J D,LANGWIG,M V,GONG X,et al. Expansion of Armatimonadota through marine sediment sequencing describes two classes with unique ecological roles[J]. ISME Communications,2023,3:64. doi: 10.1038/s43705-023-00269-x
|
[34] |
王泽铭,李传虹,马巧丽,等. 湿度盐度pH协同驱动锡林河景观疣微菌群空间异质性[J]. 微生物学报,2021,61(6):1728−1742.
WANG Zeming,LI Chuanhong,MA Qiaoli,et al. Moisture,salinity and pH co-driving spatial heterogeneity of Verrucomicrobial populations in Xilin River landscape[J]. Acta Microbiologica Sinica,2021,61(6):1728−1742.
|
[35] |
李文宝,张博尧,史玉娇,等. 乌梁素海东部流域非生长季草地土壤细菌群落结构的垂向差异[J]. 环境科学,2023,44(6):3364−6675.
LI Wenbao,ZHANG Boyao,SHI Yujiao,et al. Vertical differences in grassland bacterial community structure during non-growing season in Eastern Ulansuhai Basin[J]. Environmental Science,2023,44(6):3364−6675.
|
[36] |
喻召雄,陶先法,贾 睿,等. 2种稻虾共作模式对土壤有机氮矿化作用的影响[J]. 南方农业学报,2022,53(5):1357−1367. doi: 10.3969/j.issn.2095-1191.2022.05.018
YU Zhaoxiong,TAO Xianfa,JIA Rui,et al. Effects of two rice-shrimp co-cultural models on soil organic nitrogen mineralization[J]. Journal of Southern Agriculture,2022,53(5):1357−1367. doi: 10.3969/j.issn.2095-1191.2022.05.018
|
[37] |
YANG Y,DOU Y X,HUANG Y M,et al. Links between soil fungal diversity and plant and soil properties on the Loess Plateau[J]. Frontiers in Microbiology,2017,8:2198. doi: 10.3389/fmicb.2017.02198
|
[38] |
LYNCH M D J,NEUFELD J D. Ecology and exploration of the rare biosphere[J]. Nature Reviews Microbiology,2015,13(4):217−229. doi: 10.1038/nrmicro3400
|
[39] |
ZHENG H,LIU Y,CHEN Y,et al. Short-term warming shifts microbial nutrient limitation without changing the bacterial community structure in an alpine timberline of the eastern Tibetan Plateau[J]. Geoderma,2020,360:113985. doi: 10.1016/j.geoderma.2019.113985
|
[40] |
YUAN M M,GUO X,WU L W,et al. Climate warming enhances microbial network complexity and stability[J]. Nature Climate Change,2021,11(4):343−357. doi: 10.1038/s41558-021-00989-9
|
[41] |
JIANG Y L,SONG H F,LEI Y B,et al. Distinct co-occurrence patterns and driving forces of rare and abundant bacterial subcommunities following a glacial retreat in the eastern Tibetan Plateau[J]. Biology and Fertility of Soils,2019,55(4):351−364. doi: 10.1007/s00374-019-01355-w
|
[42] |
邢肖毅,倪 绯,张亚丽,等. 增温对土壤丰富和稀有微生物的差异性影响[J]. 环境科学与技术,2022,45(5):70−76.
XING Xiaoyi,NI Fei,ZHANG Yali,et al. Effects of warming on diversity of abundant and rare microbial communities in soil[J]. Environmental Science & Technology,2022,45(5):70−76.
|
[43] |
FAN K K,WEISENHORN P,GILBERT J A,et al. Soil pH correlates with the co-occurrence and assemblage process of diazotrophic communities in rhizosphere and bulk soils of wheat fields[J]. Soil Biology & Biochemistry,2018,121:185−192.
|
[44] |
PAN C C,FENG Q,LI Y L,et al. Rare soil bacteria are more responsive in desertification restoration than abundant bacteria[J]. Environmental Science and Pollution Research,2022,29(22):33323−33334. doi: 10.1007/s11356-021-16830-x
|
[1] | LI Guofu, JI Changjiang, LI Junjun, ZHANG Jianghua, TANG Jie, ZHAO Jinbin, SHAO Xianhua. Technology and application of coal and CBM co-mining in east fifth panel of sihe mine[J]. COAL SCIENCE AND TECHNOLOGY, 2025, 53(3): 291-303. DOI: 10.12438/cst.2025-0108 |
[2] | LIU Xiaomin, WANG Zhenyu, LIU Tingxi, WANG Wenjuan, YANG Yaotian, WANG Wenguang. Study on influencing factors of coal-water coordinated co-mining in coal resource rich area[J]. COAL SCIENCE AND TECHNOLOGY, 2024, 52(S2): 162-174. DOI: 10.12438/cst.2023-1374 |
[3] | XING Yanyang, DING Hua, BAI Xiangfei, HE Jin. Research progress on the distribution and occurrence characteristics of rare earth elements in coal and coal-fired products[J]. COAL SCIENCE AND TECHNOLOGY, 2024, 52(3): 269-282. DOI: 10.12438/cst.2023-1165 |
[4] | LI Shugang, ZHANG Jingfei, LIN Haifei, DING Yang, BAI Yang, ZHOU Yuxuan, ZHU BING, DAI Zheng. Thoughts on the development path of coal and gas co-mining technology in dual carbon strategy[J]. COAL SCIENCE AND TECHNOLOGY, 2024, 52(1): 138-153. DOI: 10.12438/cst.2023-1689 |
[5] | XU Chao, YANG Tong, WANG Kai, WU Shimin, FU Qiang, ZHOU Aitao. Knowledge map analysis of coal and gas co-mining based on Citespace[J]. COAL SCIENCE AND TECHNOLOGY, 2023, 51(S1): 86-95. DOI: 10.13199/j.cnki.cst.2022-0971 |
[6] | WANG Biru, JIA Li, WANG Yanlin, CHENG Peng, GUO Jinrong, ZHANG Liu, JIN Yan. Research on co-combustion behavior of sewage sludge and coal slime[J]. COAL SCIENCE AND TECHNOLOGY, 2023, 51(5): 284-293. DOI: 10.13199/j.cnki.cst.2021-0940 |
[7] | YAO Qiangling, TANG Chuanjin, LIU Zichang. Analysis of coal and water co-mining in ecologically fragile mining areas in Western China[J]. COAL SCIENCE AND TECHNOLOGY, 2021, 49(12): 225-232. |
[8] | ZHAO Mingkun, WANG Haiquan, XU Jun, ZHANG Yihan. Study on occurrence features of rare earth elements from Seam B1 in Henan Province[J]. COAL SCIENCE AND TECHNOLOGY, 2019, (2). |
[9] | LI Yaping, GUO Hongguang, HAN Qing, ZHANG Yiwen, LI Xingfeng. Experimental research on enhancing biogenic methane production in coal seam by co-degradation of rice straw and coal[J]. COAL SCIENCE AND TECHNOLOGY, 2018, (4). |
[10] | Wang Ruikun Zhao Zhenghui Yin Qianqian, . Effects of low temperature thermal-alkaline modification on co-slurrying performances of sludge and coal[J]. COAL SCIENCE AND TECHNOLOGY, 2017, (2). |