Analysis of the difference between weathered coal and lignite in the conversion of biomethane
-
摘要:
为查明风化煤与褐煤生物甲烷生成量差别的内在原因，分别选取风化煤和褐煤，以富集驯化的菌液为菌种来源，通过生物产气模拟，红外光谱、X射线光电子能谱、16srRNA测试、扫描电镜测试来分析风化煤与褐煤生物产气量差异性的内在机制。结果表明：褐煤的生物产气潜力（7.63 mL/g）大于风化煤（3.24 mL/g）；褐煤相比于风化煤，在产气过程中各类基团脱落明显，芳香类物质更多的被转化为小分子量的其它物质，同时酚碳或醚碳（C—O）氧化更加明显，有利于形成更多的小分子有机酸类物质；褐煤相较于风化煤在生物产气过程中表面侵蚀较严重，出现了较多的孔裂隙，使得有机质能够从这些孔隙中析出，有利于微生物进一步利用产气；褐煤中细菌和古菌多样性低于风化煤，但主要功能菌群占比较大，细菌中Macellibacteroides属和Lysinibacillus属占比达68.05%，古菌中Methanosarcina属和Methanobacterium属占比达89.99%，远大于风化煤，对煤中有机质的降解利用起到积极作用，能为后续产甲烷菌提供原料，因此褐煤更有利于被微生物利用产生物甲烷；风化煤的甲烷代谢类型为甲基类营养性区别于褐煤的氢营养型；在褐煤中微生物能够更好的利用乙酸作为碳源、葡萄糖和糖原能够被有效降解为丙酮酸，氧化（还原性）强，并产生供生化反应所需的ATP。研究结果为风化煤与褐煤产气差异机制提供理论参考，为今后风化煤与褐煤生物甲烷化利用提供现实意义。
Abstract:In order to find out the internal reasons for the difference in biological methane production between weathered coal and lignite, weathered coal and lignite were selected respectively, and the enriched and domesticated bacterial liquid was used as the source of bacteria. The internal mechanism of the difference in biological gas production between weathered coal and lignite was analyzed by biological gas production simulation, infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and 16 S rRNA test. The results showed that the biogas production potential of lignite (7.63 mL/g) was higher than that of weathered coal (3.24 mL/g). Compared with weathered coal, in the process of gas production, various groups of lignite fall off obviously, aromatic substances are more converted into other substances with small molecular weight, and phenolic carbon or ether carbon (C—O) oxidation is more obvious, which is conducive to the formation of more small molecular organic acids. Compared with weathered coal, lignite has more serious surface erosion and more pore cracks in the process of biological gas production. The diversity of bacteria and archaea in lignite is lower than that in weathered coal, but the main functional flora accounts for a large proportion. The proportion of Macellibacteroides and Lysinibacillus in bacteria reaches 68.05%, and the proportion of Methanosarcina and Methanobacterium in archaea reaches 89.99%, which is much larger than that of weathered coal. It plays a positive role in the degradation and utilization of organic matter in coal and can provide raw materials for subsequent methanogens. Therefore, lignite is more conducive to the utilization of microbial products to produce methane. Methane metabolism type of weathered coal is methyl nutrition hydrogen nutrition type different from lignite. Microorganisms in lignite can make better use of acetic acid as a carbon source, glucose and glycogen can be effectively degraded into pyruvate, strong oxidation (reduction), and produce ATP for biochemical reactions. The research results provide a theoretical reference for the mechanism of gas production difference between weathered coal and lignite, and provide practical significance for the future biomethanation utilization of weathered coal and lignite.
-
Keywords:
- biomethane /
- weathered coal /
- lignite /
- physical and chemical characteristics /
- microflora changes
-
-
![]()
图 2 褐煤和风化煤产气前后红外光谱特征
Figure 2. Infrared spectral characteristics of lignite and weathered coal gas production peak
![]()
图 3 生物产气高峰期褐煤与风化煤C（1s）的XPS拟合谱图
Figure 3. XPS fitting spectra of lignite and weathered coal C (1s) during biogas production peak
![]()
图 4 产气高峰期褐煤与风化煤扫描电镜图
Figure 4. SEM images of lignite and weathered coal at the peak of gas production
![]()
图 5 菌群多样性分析古菌韦恩图细菌韦恩图
Figure 5. Analysis of bacterial diversity Archaeal Venn diagram Bacterial Venn diagram
![]()
图 6 褐煤与风化煤产气高峰期菌群组成及丰度
Figure 6. Composition and abundance of bacteria in lignite and weathered coal gas production peak
![]()
图 7 风化煤与褐煤产气高峰期代谢通路统计
Figure 7. Statistics of metabolic pathways during the peak gas production of weathered coal and lignite
表 1 褐煤和风化煤工业分析和元素分析
Table 1 Proximate analysis and ultimate analysis of weathered coal and lignite
煤样 工业分析/% 元素分析/% Mad Aad Vad FCad Cdaf Hdaf Odaf Ndaf Sdaf 褐煤 7.46 10.71 44.58 37.25 40.43 4.40 21.47 0.40 1.34 风化煤 12.24 30.25 22.56 34.95 37.36 3.13 58.86 0.65 0 
下载: 导出CSV
表 2 风化煤与褐煤生物产气数据
Table 2 Biogas production data of weathered coal and lignite
样品 底物
质量/g有机质
含量/g累计产
气量/mLCH4体积
分数/%CH4生成量/
(mL·g−1)风化煤 20 11.502 138±5 27.02 3.24 褐煤 20 16.366 234±6 53.36 7.63
下载: 导出CSV
表 3 不同赋存形态有机碳的相对含量
Table 3 Relative contents of organic carbon in different forms
样品 C—C、C—H相对含量/% C—O相对含量/% C=O相对含量/% O—C=O相对含量/% 风化煤 89.49 3.91 3.08 3.52 褐煤 76.92 8.15 7.42 7.51
下载: 导出CSV
表 4 不同底物下菌株的多样性指数分析
Table 4 Analysis of diversity index of strains under different substrates
菌株 样品 Chao1 Species Shannon 古菌 褐煤 51.91 51.81 2.16 风化煤 63.88 63.82 2.24 细菌 褐煤 679.88 674.53 4.83 风化煤 686.87 685.71 5.05
下载: 导出CSV
-
[1] 刘淑琴,刘 欢,纪雨彤,等. 深部煤炭地下气化制氢碳排放核算及碳减排潜力分析[J]. 煤炭科学技术,2023,51(1):531−541. LIU Shuqin,LIU Huan,JI Yutong,et al. Carbon emission accounting and carbon reduction analysis for deep coal underground gasification to hydrogen[J]. Coal Science and Technology,2023,51(1):531−541.
[2] 赵 浩. 露天煤矿高质量安全发展形势分析与对策措施[J]. 煤矿安全,2022,53(7):251−256. ZHAO Hao. Analysis of development situation and countermeasures of high quality safety in open-pit coal mines[J]. Safety in Coal Mines,2022,53(7):251−256.
[3] 郗小明,秦 超,罗进成,等. 煤焦化与多元料浆气化优化集成制取合成甲醇原料气优势分析[J]. 煤化工,2018,46(1):30−33. doi: 10.3969/j.issn.1005-9598.2018.01.008 CHI Xiaoming,QIN Chao,LUO Jincheng,et al. Advantage analysis of optimal-integrated system of coal coking with multi-component slurry gasification to feed gas for methanol synthesis[J]. Coal Chemical Industry,2018,46(1):30−33. doi: 10.3969/j.issn.1005-9598.2018.01.008
[4] 梅艳钢,王志青,张 郃,等. 碱金属迁移对煤焦气化反应性的原位探究[J]. 燃料化学学报,2021,49(6):735−741. doi: 10.1016/S1872-5813(21)60031-2 MEI Yangang,WANG Zhiqing,ZHANG He,et al. In-situ study of effect of migrating alkali metals on gasification reactivity of coal char[J]. Journal of Fuel Chemistry and Technology,2021,49(6):735−741. doi: 10.1016/S1872-5813(21)60031-2
[5] 邹 涛,朱春鹏,张 瑜,等. 含油污泥与配合煤共热解煤焦的微观结构与气化反应特性[J]. 燃料化学学报,2020,48(2):137−143. ZHOU Tao,ZHU Chunpeng,ZHANG Yu,et al. Characteristics of microstructures and gasification reactivity of co-pyrolysis coal with oily sludge and blended coal[J]. Journal of Fuel Chemistry and Technology,2020,48(2):137−143.
[6] 司 硕. 生物钙脱硝技术在工业层燃炉中的应用[J]. 煤炭加工与综合利用,2021(10):93−96. doi: 10.16200/j.cnki.11-2627/td.2021.10.022 SI Shuo. Application of biological calciumdenitration technology in layer burning industrial boiler[J]. Coal Processing & Comprehensive Utilization,2021(10):93−96. doi: 10.16200/j.cnki.11-2627/td.2021.10.022
[7] 龚艳艳. 煤燃烧过程中燃料型NOx生成及还原机理研究进展[J]. 煤质技术,2022,37(3):9−23. doi: 10.3969/j.issn.1007-7677.2022.03.002 GONG Yanyan. Research progress on formation and reduction mechanism of fuel-NOx during coal combustion[J]. Coal Quality Technology,2022,37(3):9−23. doi: 10.3969/j.issn.1007-7677.2022.03.002
[8] 朱振武,禚玉群. 煤炭洗选中有害痕量元素的迁移与脱除[J]. 煤炭学报,2016,41(10):2434−2440. doi: 10.13225/j.cnki.jccs.2016.8003 ZHU Zhenwu,ZHUO Yuqun. Migration and removal of toxic trace elements during coal washing[J]. Journal of China Coal Society,2016,41(10):2434−2440. doi: 10.13225/j.cnki.jccs.2016.8003
[9] 纪 栋,姚志松,张 陈,等. 餐厨垃圾厌氧发酵产沼气过程中的微生物群落结构解析[J]. 太阳能学报,2022,43(9):354−362. doi: 10.19912/j.0254-0096.tynxb.2020-1403 JI Dong,YAO Zhisong,ZHANG Chen,et al. Analysis of microbial community structure in biogas production by anaerobic fermentation of kitchen waste[J]. Acta Energiae Solaris Sinica,2022,43(9):354−362. doi: 10.19912/j.0254-0096.tynxb.2020-1403
[10] 曹 麒,何雨恒,卓桂华,等. 高温条件下初始pH值对污泥-餐厨垃圾联合厌氧发酵产氢余物产CH4的影响[J]. 环境工程,2022,40(9):150−157. CAO Qi,HE Yuheng,ZHUO Guihua,et al. Effect on initial pH value on methane production from residue after anaerobic co-fermentative hudrogen production of sewage sludge and food waste under thermophilic operation[J]. Environmental Engineering,2022,40(9):150−157.
[11] 李 洋, 唐书恒, 陈 健, 等. 影响煤生物气化的物化特征及煤预处理的研究进展[J]. 微生物学报, 2022, 62(6): 2328−2339. LI Yang, TANG Shuheng, CHEN Jian, et al. Physicochemical characteristics affecting biological coal gasification and coal pretreatment[J]. Acta Microbiologica Sinica, 2022, 62(6): 2328−2339.
[12] 李春全,王丝蒂,汪欣林,等. 风化煤基催化材料的制备及其活化PMS降解苯酚[J]. 煤炭学报,2022,47(5):2067−2077. doi: 10.13225/j.cnki.jccs.2022.0181 LI Chunquan,WANG Sidi,WANG Xinlin,et al. Preparation of weathered coal-based catalytic material and its performance in phenol degradation by activating peroxymonosulfate[J]. Journal of China Coal Society,2022,47(5):2067−2077. doi: 10.13225/j.cnki.jccs.2022.0181
[13] 郭红玉,李赛赛,沈 野,等. 不同风化程度煤的结构与生物产气效果的变化特征[J]. 安全与环境学报,2022,22(3):1510−1517. doi: 10.13637/j.issn.1009-6094.2021.0556 GUO Hongyu,LI Saisai,SHEN Ye,et al. Variation characteristics of the structure and biological gas production effect of coal with different weathering degrees[J]. Journal of Safety and Environment,2022,22(3):1510−1517. doi: 10.13637/j.issn.1009-6094.2021.0556
[14] 郭红玉,李云嵩,李贤忠,等. 以风化煤为底物制取生物甲烷的潜力分析[J]. 煤炭学报,2020,45(3):1187−1194. doi: 10.13225/j.cnki.jccs.2019.0278 GUO Hongyu,LI Yunsong,LI Xianzhong,et al. Potential analysis of biological methane production with weathered coal[J]. Journal of China Coal Society,2020,45(3):1187−1194. doi: 10.13225/j.cnki.jccs.2019.0278
[15] 刘 薇,李啸宇,何 环,等. 硫酸盐还原菌Desulfotomaculum reducens ZTS1厌氧降解昭通褐煤[J]. 微生物学报,2021,61(6):1610−1620. LIU Wei,LI Xiaoyu,HE Huan,et al. Anaerobic degradation of Zhaotong lignite by sulfate reducing bacterium Desulfotomaculum reducens ZTS1[J]. Acta Microbiologica Sinica,2021,61(6):1610−1620.
[16] 张怀文,姚义清,谢昌文. 不同联合预处理对褐煤厌氧发酵产甲烷的影响[J]. 煤田地质与勘探,2021,49(4):162−169. doi: 10.3969/j.issn.1001-1986.2021.04.019 ZHANG Huaiwen,YAO Yiqing,XIE Changwen. Effects of different combined pretreatments on biogenic methane production by anaerobic digestion of lignite[J]. Coal Geology & Exploration,2021,49(4):162−169. doi: 10.3969/j.issn.1001-1986.2021.04.019
[17] 夏大平, 刘春兰, 陈振宏, 等. 不同煤阶煤孔隙结构与煤制生物甲烷的相互影响[J]. 煤炭转化, 2023, 46(1): 27−38. XIA Daping, LIU Chunlan, CHEN Zhenhong, et al. The interaction between pore structure of differennt rank coals and coal-to-biomethane[J]. Coal Conversion, 2023, 46(1): 27−38.
[18] 李兴凤,郭红光,张亦雯,等. NaOH预处理对无烟煤生物甲烷转化的影响[J]. 煤矿安全,2019,50(11):6−9. doi: 10.13347/j.cnki.mkaq.2019.11.002 LI Xingfeng,GUO Hongguang,ZHANG Yiwen,et al. Effect of NaOH pretreatment on biomethane conversion in anthracite[J]. Safety in Coal Mines,2019,50(11):6−9. doi: 10.13347/j.cnki.mkaq.2019.11.002
[19] 侯 彪,王子升,周艺璇,等. 不同煤阶煤制生物甲烷的代谢功能差异性研究[J]. 煤炭科学技术,2021,49(12):119−126. doi: 10.3969/j.issn.0253-2336.2021.12.mtkxjs202112015 HOU Biao,WANG Zisheng,ZHOU Yixuan,et al. Difference of metabolic functions in biomethane production from different rank coals[J]. Coal Science and Technology,2021,49(12):119−126. doi: 10.3969/j.issn.0253-2336.2021.12.mtkxjs202112015
[20] SU Xianbo,ZHAO Weizhong,XIA Daping,et al. The diversity of hydrogen-producing bacteria and methanogens within an in situ coal seam[J]. Biotechnology for Biofuels,2018,11(1):245−263. doi: 10.1186/s13068-018-1237-2
[21] 赵伟仲. 煤层本源菌制取生物甲烷的实验研究[D]. 焦作: 河南理工大学, 2019. ZHAO Weizhong. Experimental research on biomethane production from microorganism in situ coal seam[D]. Jiaozuo: Henan Polytechnic University, 2019.
[22] PETERSEN Henriki,ROSENBERG Per,NYTOFT,Hansp. Oxygen groups in coals and alginite-rich kerogen revisited[J]. International Journal of Coal Geology,2008,74(2):93−113. doi: 10.1016/j.coal.2007.11.007
[23] 贾廷贵,李 璕,曲国娜,等. 不同变质程度煤样化学结构特征FTIR表征[J]. 光谱学与光谱分析,2021,41(11):3363−3369. JIA Tinggui,LI Xun,QU Guona,et al. FTIR characterization of chemical structures characteristics of coal samples with different metamorphic degrees[J]. Spectroscopy and Spectral Analysis,2021,41(11):3363−3369.
[24] 何 鑫,王文峰,章新喜,等. 低阶煤显微组分含氧官能团的分布特征与差异[J]. 煤炭学报,2021,46(9):2804−2812. doi: 10.13225/j.cnki.jccs.FX21.0695 HE Xin,WANG Wenfeng,ZHANG Xinxi,et al. Distribution characteristics and differences of oxygen-containing functional groups in macerals of low rank coal[J]. Journal of China Coal Society,2021,46(9):2804−2812. doi: 10.13225/j.cnki.jccs.FX21.0695
[25] YIN Yalin,GAO Chongyang,ZHAO Yangguo,et al. Electricity generation and dynamics characteristics of microbial community of microbial fuel cells started up with mixture of aerobic/anaerobic sludge[J]. Acta Microbiologica Sinica,2014,54(12):1471−1480.
[26] LI Jinda,YI Mengwen,WANG Lili,et al. Isolation and screening of a strain of anaerobic electricity-producing phenol degrading bacteria and biological characteristics research[J]. Harbin University of Science and Technology.,2020,25(2):150−159.
[27] GUU Hngyu,ZHANG Minglu,DONG Zhiwei,et al. The mechanisms of biogenic methane metabolism by synergistic biodegradation of coal and corn straw[J]. Bioresource Technology,2020,298:122577. doi: 10.1016/j.biortech.2019.122577
[28] ZOU Hui,GAO Ming,YU Miao,et al. Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation:Methanogenic pathway and microbial community analyses[J]. Bioresource Technology,2020,297:122450. doi: 10.1016/j.biortech.2019.122450
[29] ZHAO Zhiqiang,WANG Jianfeng,LI Yang,et al. Why do DIETers like drinking:metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol[J]. Water Research,2020,171:115425. doi: 10.1016/j.watres.2019.115425
[30] LIU Chuanqi, SUN Dezhi, ZHAO Zhiqiang, et al. Methanothrix enhances biogas upgrading in microbial electrolysis cell via direct electron transfer[J]. Bioresource Technology, 2019, 291: 121877.
[31] SAHA Shouvik, KURADE Mayurb, Ha Geeonsoo, et al. Syntrophic metabolism facilitates Methansarcina-led methanation in the anaerobic digestion of lipidic slaughterhouse waste[J]. Bioresource Technology, 2021, 335: 125250.
[32] WANG Dexin, HAN Yuxing, HAN Hongjun, et al. New insights into enhanced anaerobic degradation of Fischer-Tropsch wastewater with the assistance of magnetite[J]. Bioresource Technology, 2018, 257: 147−156.
[33] POBEHEIM Herbert. , MUNK Bernhard. , LINDORFER Harold, et al. Impact of nickel and cobalt on biogas production and process stability during semi-continuous anaerobic fermentation of a model substrate for maize silage[J]. Water Research. 2011, 45: 781−787.
[34] GUO Hao, HUA Junjie, CHENG Jun, et al. Microbial electrochemistry enhanced electron transfer in lactic acid anaerobic digestion for methane production[J]. Journal of Cleaner Production, 2022, 358: 131983.
-
期刊类型引用(2)
1. 马力通，白月姝，梁宏旺，孙慎光. 预处理对褐煤非燃料化利用影响的研究进展. 化学与生物工程. 2025(03): 1-7 . 
百度学术
2. 邓军，张敏，易欣，白祖锦，王彩萍，王凯. 煤矿采空区微生物驯化及抑制煤自燃特性. 煤炭学报. 2025(01): 379-391 . 百度学术
其他类型引用(2)
计量
- 文章访问数: 84
- HTML全文浏览量: 19
- PDF下载量: 33
- 被引次数: 4
